期刊
MICROORGANISMS
卷 9, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9061315
关键词
SARS-CoV2; mRNA vaccine; health care workers; whole blood T cell assay; coordinate immunity
类别
资金
- Italian Ministry of Health
- Esselunga S.p.A. [88/2021]
The study demonstrates that the BNT162b2 mRNA vaccine can induce both humoral and cell-mediated immune responses against spike peptides in nearly all healthcare workers without previous SARS-CoV-2 infection, with a moderate inverse correlation with age in the anti-RBD response.
Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of health care workers (HCWs) who received a two-dose BNT162b2-mRNA vaccination. The serological response was evaluated by quantifying the anti-RBD and neutralizing antibodies. The cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-gamma, TNF-alpha, IL-2), produced in response to spike peptides. The BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune responses against spike peptides in virtually all HCWs without previous SARS-CoV-2 infection, with a moderate inverse relation with age in the anti-RBD response. Spike-specific T cells produced several Th1 cytokines (IFN-gamma, TNF-alpha, and IL-2), which correlated with the specific-serological response. Overall, our study describes the ability of the BNT162b2 mRNA vaccine to elicit a coordinated neutralizing humoral and spike-specific T cell response in HCWs. Assessing the dynamics of these parameters by an easy immune monitoring protocol can allow for the evaluation of the persistence of the vaccine response in order to define the optimal vaccination strategy.
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