期刊
MICROORGANISMS
卷 9, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9091855
关键词
anti-allergy; germinated brown rice fermented with lactic acid bacteria; basophil; type I hypersensitive disease; mast cell
类别
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2021R1F1A1048156]
This study investigated the effect of GBR fermented with the Pediococcus pentosaceus SP024 strain on IgE/Ag mediated passive cutaneous anaphylaxis, showing its potential as an anti-allergic agent through the regulation of protein and gene expressions in RBL-2H3 cells. The study also demonstrated the effectiveness of GBR-SP024 in reducing immune cell infiltration, mast cell degranulation, and pro-inflammatory cytokine levels in a murine model, suggesting its use as a dietary supplement or adjuvant for treating IgE-dependent allergic diseases.
In this study, the effect of GBR fermented with the Pediococcus pentosaceus SP024 strain on IgE/Ag mediated passive cutaneous anaphylaxis (PCA) was investigated. Protocatechuic acid and trans-ferulic acid levels in GBR-SP024 increased more than those in unfermented GBR, respec-tively. The inhibitory activity of GBR-SP024 on beta-hexosaminidase release and the level of proin-flammatory cytokine mRNA expression (tumor necrosis factor-alpha (TNF-alpha) and interleukin 4 (IL-4)) was observed in IgE/Ag-stimulated RBL-2H3 cells. Western blot analysis showed that GBR-SP024 significantly inhibited the phosphorylation of the linker for activation of T cell (LAT) and nuclear factor-kappa B (NF-kappa B) in IgE/Ag-stimulated RBL-2H3 cells. Further, we investigated the anti-allergic effect of GBR-SP024 using PCA murine model. The number of infiltrated immune cells and degranulated mast cells in GBR-SP024 treated dermis was lower than that in the GBR-treated mice. In addition, mRNA expression of 5-lipoxygenase (5-LOX) in the dermis of ear tissue declined in the GBR-SP024-treated group, compared to that in the GBR group. GBR-SP024 was also more effective than GBR at reducing the levels of IL-33 protein expression in IgE/Ag-stimulated BALB/c mice. Our study suggests the potential usage of GBR-SP024 as a dietary supplement or an adjuvant for treating IgE-dependent-allergic diseases.
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