期刊
MICROORGANISMS
卷 9, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9061305
关键词
dengue; endothelial cells; endocan; vascular dysfunction
类别
资金
- Consejo Nacional de Ciencia y Tecnologia (CONACyT) Fronteras de la Ciencia [2015-02-1192]
- Ciencia Basica SEP-CONACyT [256235]
- FosissCONACyT [261693]
The study found that recombinant NS1 proteins can promote the expression and release of the endothelial cell activation marker endocan, and increase its levels within cells. These findings help us to further understand the dysfunction of vascular endothelial cells in dengue virus infections.
A common hallmark of dengue infections is the dysfunction of the vascular endothelium induced by different biological mechanisms. In this paper, we studied the role of recombinant NS1 proteins representing the four dengue serotypes, and their role in promoting the expression and release of endocan, which is a highly specific biomarker of endothelial cell activation. We evaluated mRNA expression and the levels of endocan protein in vitro following the stimulation of HUVEC and HMEC-1 cell lines with recombinant NS1 proteins. NS1 proteins increase endocan mRNA expression 48 h post-activation in both endothelial cell lines. Endocan mRNA expression levels were higher in HUVEC and HMEC-1 cells stimulated with NS1 proteins than in non-stimulated cells (p < 0.05). A two-fold to three-fold increase in endocan protein release was observed after the stimulation of HUVECs or HMEC-1 cells with NS1 proteins compared with that in non-stimulated cells (p < 0.05). The blockade of Toll-like receptor 4 (TLR-4) signaling on HMEC-1 cells with an antagonistic antibody prevented NS1-dependent endocan production. Dengue-infected patients showed elevated serum endocan levels (>= 30 ng/mL) during early dengue infection. High endocan serum levels were associated with laboratory abnormalities, such as lymphopenia and thrombocytopenia, and are associated with the presence of NS1 in the serum.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据