4.7 Article

Selected Kefir Water from Malaysia Attenuates Hydrogen Peroxide-Induced Oxidative Stress by Upregulating Endogenous Antioxidant Levels in SH-SY5Y Neuroblastoma Cells

期刊

ANTIOXIDANTS
卷 10, 期 6, 页码 -

出版社

MDPI
DOI: 10.3390/antiox10060940

关键词

kefir water; neurodegeneration; oxidative stress; antioxidant; neuroprotection

资金

  1. Ministry of Education (MOE) through Fundamental Research Grant Scheme [FRGS-MRSA/1/2018/SKK10/UPM/02/1]
  2. Universiti Putra Malaysia (UPM)

向作者/读者索取更多资源

The study found that Kefir B in kefir water exhibited the highest antioxidant and anti-apoptotic activities, providing significant cell protection and maintaining cellular morphology. The results suggest that kefir water can exert its antioxidative, anti-apoptotic, and neuroprotective effects through upregulating enzymes like SOD and catalase.
Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six different kefir water samples were analysed by total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2 '-diphenyl-1-picrylhydrazyl radical (DPPH) assays, whereas the anti-apoptotic activity on hydrogen peroxide (H2O2) induced SH-SY5Y cells was examined using MTT, AO/PI double staining, and PI/Annexin V-FITC assays. The surface and internal morphological features of SH-SY5Y cells were studied using scanning and transmission electron microscopy. The results indicate that Kefir B showed the higher TPC (1.96 +/- 0.54 mu g GAE/mu L), TFC (1.09 +/- 0.02 mu g CAT eq/mu L), FRAP (19.68 +/- 0.11 mM FRAP eq/50 mu L), and DPPH (0.45 +/- 0.06 mg/mL) activities compared to the other kefir samples. The MTT and PI/Annexin V-FITC assays showed that Kefir B pre-treatment at 10 mg/mL for 48 h resulted in greater cytoprotection (97.04%), and a significantly lower percentage of necrotic cells (7.79%), respectively. The Kefir B pre-treatment also resulted in greater protection to cytoplasmic and cytoskeleton inclusion, along with the conservation of the surface morphological features and the overall integrity of SH-SY5Y cells. Our findings indicate that the anti-oxidative, anti-apoptosis, and neuroprotective effects of kefir were mediated via the upregulation of SOD and catalase, as well as the modulation of apoptotic genes (Tp73, Bax, and Bcl-2).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据