期刊
GLIA
卷 64, 期 12, 页码 2154-2165出版社
WILEY
DOI: 10.1002/glia.23049
关键词
olfactory ensheathing cells; BDNF; TRPCs; migration; calcium
资金
- Natural Science Foundation of Zhejiang Province [LY15C090006]
- Science and Technology Planning Project of Zhejiang Province [2013C33167]
- National Natural Science Foundation of China [81371350, 81571190]
Olfactory ensheathing cells (OECs) are a unique type of glial cells with axonal growth-promoting properties in the olfactory system. Organized migration of OECs is essential for neural regeneration and olfactory development. However, the molecular mechanism of OEC migration remains unclear. In the present study, we examined the effects of brain-derived neurotrophic factor (BDNF) on OEC migration. Initially, the scratch migration assay, the inverted coverslip and Boyden chamber migration assays showed that BDNF could promote the migration of primary cultured OECs. Furthermore, BDNF gradient attracted the migration of OECs in single-cell migration assays. Mechanistically, TrkB receptor expressed in OECs mediated BDNF-induced OEC migration, and BDNF triggered calcium signals in OECs. Finally, transient receptor potential cation channels (TRPCs) highly expressed in OECs were responsible for BDNF-induced calcium signals, and required for BDNF-induced OEC migration. Taken together, these results demonstrate that BDNF promotes the migration of cultured OECs and an unexpected finding is that TRPCs are required for BDNF-induced OEC migration.
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