期刊
ANTIOXIDANTS
卷 10, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/antiox10071155
关键词
chronic kidney disease; oxidative stress; polymorphonuclear leukocytes; mononuclear leukocytes; hemodialysis; peritoneal dialysis
资金
- Instituto de Salud Carlos III (European Regional Development Fund A way to make Europe) [PI17/01029, PI20/01321]
- Contratos FiBio-HCR asociados a Proyectos de Investigacion (Comunidad de Madrid y Fondo Social Europeo) [PEJ-2020-AI/BMD-18141]
- IRYCIS, Madrid, Spain
- Universidad de Alcala
The study reveals the significant role of oxidative stress in CKD patients, with dialysis procedures helping to maintain the GSSG/GSH balance in PMNs. PD patients showed higher oxidative stress than HD patients, particularly in MNs. The assessment of redox state parameters in PMNs and MNs may serve as potential biomarkers for CKD progression.
Oxidative stress plays a key role in the pathophysiology of chronic kidney disease (CKD). Most studies have investigated peripheral redox state focus on plasma, but not in different immune cells. Our study analyzed several redox state markers in plasma and isolated peripheral polymorphonuclear (PMNs) and mononuclear (MNs) leukocytes from advanced-CKD patients, also evaluating differences of hemodialysis (HD) and peritoneal dialysis (PD) procedures. Antioxidant (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH)) and oxidant parameters (xanthine oxidase (XO), oxidized glutathione (GSSG), malondialdehyde (MDA)) were assessed in plasma, PMNs and MNs from non-dialysis-dependent-CKD (NDD-CKD), HD and PD patients and healthy controls. Increased oxidative stress and damage were observed in plasma, PMNs and MNs from NDD-CKD, HD and PD patients (increased XO, GSSG and MDA; decreased SOD, CAT, GPX and GSH; altered GSSG/GSH balance). Several oxidative alterations were more exacerbated in PMNs, whereas others were only observed in MNs. Dialysis procedures had a positive effect on preserving the GSSG/GSH balance in PMNs. Interestingly, PD patients showed greater oxidative stress than HD patients, especially in MNs. The assessment of redox state parameters in PMNs and MNs could have potential use as biomarkers of the CKD progression.
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