期刊
ANTIOXIDANTS
卷 10, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/antiox10060919
关键词
WrbA; quinone oxidoreductase activity; biofilm formation; oxidative stress; mature biofilm; Escherichia coli
资金
- Fondazione Cariplo, VOLAC-Valorization of Live Oil Wastes for Sustainable Production of Biocide-Free Antibiofilm Compounds [2017-0977]
The study demonstrates the important role of WrbA in biofilm development, with its absence affecting biofilm formation and rendering the cells sensitive to reactive oxygen species. Salicylic and cinnamic acids act as antibiofilm agents by inhibiting WrbA's enzyme activity for the first time.
The effects of natural compounds on biofilm formation have been extensively studied, with the goal of identifying biofilm formation antagonists at sub-lethal concentrations. Salicylic and cinnamic acids are some examples of these compounds that interact with the quinone oxidoreductase WrbA, a potential biofilm modulator and an antibiofilm compound biomarker. However, WrbA's role in biofilm development is still poorly understood. To investigate the key roles of WrbA in biofilm maturation and oxidative stress, Escherichia coli wild-type and increment wrbA mutant strains were used. Furthermore, we reported the functional validation of WrbA as a molecular target of salicylic and cinnamic acids. The lack of WrbA did not impair planktonic growth, but rather affected the biofilm formation through a mechanism that depends on reactive oxygen species (ROS). The loss of WrbA function resulted in an ROS-sensitive phenotype that showed reductions in biofilm-dwelling cells, biofilm thickness, matrix polysaccharide content, and H2O2 tolerance. Endogenous oxidative events in the mutant strain generated a stressful condition to which the bacterium responded by increasing the catalase activity to compensate for the lack of WrbA. Cinnamic and salicylic acids inhibited the quinone oxidoreductase activity of purified recombinant WrbA. The effects of these antibiofilm molecules on WrbA function was proven for the first time.
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