4.7 Article

Insights into the Interaction of Lysosomal Amino Acid Transporters SLC38A9 and SLC36A1 Involved in mTORC1 Signaling in C2C12 Cells

期刊

BIOMOLECULES
卷 11, 期 9, 页码 -

出版社

MDPI
DOI: 10.3390/biom11091314

关键词

amino acid transporter; lysosome; leucine; mTORC1 signaling pathway

资金

  1. National Key R&D Program of China [2017YFD0502000]
  2. National Natural Science Foundation of China [31301946, 31402051]
  3. Fundamental Research Funds for the Central Universities [2662020DKPY012]
  4. Research Funds for Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding [KLAEMB-2019-01]
  5. Key R&D projects of Hubei Province [2020BBB069]

向作者/读者索取更多资源

The study reveals that leucine can increase the expressions of SLC38A9 and SLC36A1, leading to mTORC1 activation, and SLC38A9 interacts with SLC36A1 to enhance each other's expression levels and locations on the lysosomal surface. Interacting proteins of SLC38A9 in C2C12 cells are involved in amino acid sensing mechanism, mTORC1 signaling pathway, and protein synthesis, providing a resource for future investigations of skeletal muscle mass.
Amino acids are critical for mammalian target of rapamycin complex 1 (mTORC1) activation on the lysosomal surface. Amino acid transporters SLC38A9 and SLC36A1 are the members of the lysosomal amino acid sensing machinery that activates mTORC1. The current study aims to clarify the interaction of SLC38A9 and SLC36A1. Here, we discovered that leucine increased expressions of SLC38A9 and SLC36A1, leading to mTORC1 activation. SLC38A9 interacted with SLC36A1 and they enhanced each other's expression levels and locations on the lysosomal surface. Additionally, the interacting proteins of SLC38A9 in C2C12 cells were identified to participate in amino acid sensing mechanism, mTORC1 signaling pathway, and protein synthesis, which provided a resource for future investigations of skeletal muscle mass.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据