期刊
BIOMOLECULES
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/biom11081177
关键词
metabolism; glycolysis; mitochondria; cell culture; mitochondrial networks; oxidative phosphorylation
资金
- Natural Science and Engineering Research Council (NSERC) Discovery Grant [RGPIN-2015-05645]
- Ontario Graduate Studies (OGS) scholarship
The study shows that different cell culture media composition and oxygen levels can significantly affect mitochondrial abundance, network structure, and cellular energy metabolism in cancer cells. Cells cultured in DMEM generally exhibit higher glycolytic activity and lower oxygen consumption rates.
Cancer cell culture is routinely performed under superphysiologic O-2 levels and in media such as Dulbecco's Modified Eagle Medium (DMEM) with nutrient composition dissimilar to mammalian extracellular fluid. Recently developed cell culture media (e.g., Plasmax, Human Plasma-Like Medium (HPLM)), which are modeled on the metabolite composition of human blood plasma, have been shown to shift key cellular activities in several cancer cell lines. Similar effects have been reported with respect to O-2 levels in cell culture. Given these observations, we investigated how media composition and O-2 levels affect cellular energy metabolism and mitochondria network structure in MCF7, SaOS2, LNCaP, and Huh? cells. Cells were cultured in physiologic (5%) or standard (18%) O-2 levels, and in physiologic (Plasmax) or standard cell culture media (DMEM). We show that both O-2 levels and media composition significantly affect mitochondrial abundance and network structure, concomitantly with changes in cellular bioenergetics. Extracellular acidification rate (ECAR), a proxy for glycolytic activity, was generally higher in cells cultured in DMEM while oxygen consumption rates (OCR) were lower. This effect of media on energy metabolism is an important consideration for the study of cancer drugs that target aspects of energy metabolism, including lactate dehydrogenase activity.
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