期刊
VACCINES
卷 9, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/vaccines9050534
关键词
swine; bacteria; disease; vaccine; Glaesserella parasuis
资金
- Ministry of Economy of Spain (MINECO) [AGL2016-77361-R]
- Ministry of Economy of Spain (MINECO) of the Spanish government
Vaccination of sows with a protein fragment from virulent G. parasuis resulted in improved immune response, increased weight gain, and lower body temperature in their offspring after challenge. Additionally, higher levels of surfactant protein D were observed in bronchoalveolar lavage of piglets from vaccinated sows, indicating a stronger immune response.
Glaesserella (Haemophilus) parasuis, an early colonizer of the nasal cavity in piglets, is a highly heterogeneous species, comprising both commensal and virulent strains. Virulent G. parasuis strains can cause fibrinous polyserositis called Glasser's disease. Colostrum is a source of passive immunity for young piglets. When vaccinating sows, protective antibodies are transferred to their offspring through the colostrum. Here, sow vaccination was performed with a protein fragment, F4, from the outer membrane trimeric autotransporters VtaAs exclusively found in virulent G. parasuis. Piglets were allowed to suckle for 3 weeks, following which a challenge with two virulent strains of G. parasuis was performed. A group of nonvaccinated sows and their piglets were included as a control. Antibodies against F4 were confirmed using ELISA in the vaccinated sows and their offspring before the G. parasuis challenge. Compared to the control group, F4-vaccination also resulted in an increased level of serum TGF-beta both in vaccinated sows and in their offspring at early time points of life. After the challenge, a lower body temperature and a higher weight were observed in the group of piglets from vaccinated sows. One piglet from the non-vaccinated group succumbed to the infection, but no other significant differences in clinical signs were noticed. At necropsy, performed 2 weeks after the virulent challenge, the level of surfactant protein D (SP-D) in bronchoalveolar lavage was higher in the piglets from vaccinated sows. Vaccination did not inhibit the nasal colonization of the piglets by the challenge strains.
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