期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.690747
关键词
single nucleotide polymorphism; gold nanoparticle; gastric cancer; solid nanopore; DNA probe
资金
- National Key Research and Development Program of China [2017YFA0205301, 2017YFA0205302]
- Key Research and Development Program of Jiangsu [BE2018732]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [YX030003]
- open research fund of Jiangsu Key Laboratory for Biosensors [51204080]
- Project of Nanjing University of Posts and Telecommunications [NY220222, 2020XZZ16]
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University [OPSKLB202012]
A novel SNP detection assay has been developed for single-nucleotide discrimination using a nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The study successfully detected the SNP of p53 gene mutation in gastric cancer at femtomolar concentration, offering high sensitivity and spatial resolution for potential applications in early diagnosis and disease prevention.
Single-nucleotide polymorphisms (SNPs) are the abundant forms of genetic variations, which are closely associated with serious genetic and inherited diseases, even cancers. Here, a novel SNP detection assay has been developed for single-nucleotide discrimination by nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The SNP of p53 gene mutation in gastric cancer has been successfully detected in the femtomolar concentration by nanopore sensing. The robust biosensing strategy offers a way for solid nanopore sensors integrated with varied nanoparticles to achieve single-nucleotide distinction with high sensitivity and spatial resolution, which promises tremendous potential applications of nanopore sensing for early diagnosis and disease prevention in the near future.
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