期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.755003
关键词
acidic xylanase; corncob; sonication; Penicillium janthinellum; xylo-oligosaccharides
资金
- National Key Research and Development Program of China [2017YFD0400206]
- National Natural Science Foundation of China [31671793, 31201449, 31830069, 31371723]
- Beijing Natural Science Foundation
- Beijing Municipal Education Commission [KZ202010011018]
- Foundation of Beijing Technology and Business University [PXM 2017 014213000036, PXM 2017_014213_000036]
A novel treatment involving enzymatic hydrolysis using an acidic xylanase coupled with ultrasound was performed to improve the xylo-oligosaccharides (XOS) yield from corncob bran. Mechanism studies indicated that ultrasonic pretreatment could reduce the beta-fold content and increase the random coil content, promoting the production of XOS. The results suggest that ultrasound can alter the structure and size of the substrate, facilitating the enzymatic reaction and enhancing the production of XOS.
A novel treatment involving enzymatic hydrolysis using an acidic xylanase coupled with ultrasound was performed to improve the xylo-oligosaccharides (XOS) yield from corncob bran. The acidic xylanase (XynB) was purified to a most suitable pH, temperature, and operational parameters for ultrasound-assisted hydrolysis were determined. A preliminary mechanistic investigation was performed through circular dichroism (CD) spectroscopy, scanning electron microscope (SEM) and a laser particle size analyzer, and the effects of ultrasound on enzyme (XynB) and substrate (corncob bran) were assessed. The results show that the maximum XOS yield was 20.71% when the reaction pH and temperature were 4.3 and 50 degrees C, the ultrasonic parameters were 50 kHz and 0.40 W/cm(2), which was 2.55 fold higher than that obtained using a non-ultrasound-assisted enzymatic preparation. Mechanism studies indicated that ultrasonic pretreatment could reduce the beta-fold content and increase the random coil content. Changes in structure and size of substrate were observed. The specific surface area of the XAC molecules is easy to carry out enzymatic reaction, which is beneficial to the production of XOS.
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