期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.718466
关键词
CRISPR; Cas9; off-targets; Cas9 variants; DNA repair; gene editing
资金
- iNOVA4Health
The repurposing of the CRISPR/Cas bacterial defense system as molecular tools has revolutionized gene editing, and efforts have been made since 2013 to decrease the frequency of unwanted mutations during CRISPR-based gene editing.
The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.
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