期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.698315
关键词
ovary; folliculogenesis; tissue clearing; microscopy; 3D analysis
资金
- Global Consortium for Reproductive Longevity and Equality at the Buck Institute [GCRLE-1620]
- W. M. Keck Foundation
- UCSF Program for Breakthrough Biomedical Research
- [P30-ES030284]
- [R01ES028212]
- [R01GM122902]
The ovary is essential for female reproduction and health, but studying its function in mammals faces challenges due to unique characteristics. Advances in optical tissue clearing and 3D visualization technologies have provided a platform to explore the structure of the ovary and have potential applications in treating ovarian diseases and extending female reproductive lifespan.
The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodeling during each hormonal cycle. Overcoming the limitations of traditional histology, recent advances in optical tissue clearing and three-dimensional (3D) visualization offer an advanced platform to explore the architecture of intact organs at a single cell level and reveal new relationships and levels of organization. Here we summarize the development and function of ovarian compartments that have been delineated by conventional two-dimensional (2D) methods and the limits of what can be learned by these approaches. We compare types of optical tissue clearing, 3D analysis technologies, and their application to the mammalian ovary. We discuss how 3D modeling of the ovary has extended our knowledge and propose future directions to unravel ovarian structure toward therapeutic applications for ovarian disease and extending female reproductive lifespan.
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