期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.628573
关键词
extracellular vesicle; lncRNA SNHG16; breast cancer; competing endogenous RNA; miR-892b; PPAPDC1A; bioinformatics analysis
资金
- National Natural Science Foundation of China Youth Science Foundation Project [82003116]
The study demonstrated that SNHG16 expression is significantly increased in breast cancer tissues and cells, promoting migration, invasion, and epithelial-mesenchymal transition (EMT) of breast cancer cells through extracellular vesicles (EVs). SNHG16 was shown to regulate PPAPDC1A expression by sponging miR-892b, facilitating the progression of breast cancer. Additionally, enhanced miR-892b expression partially mitigated the effects of SNHG16 or EVs on breast cancer cell migration, invasion, and EMT.
Breast cancer (BC) represents the most commonly diagnosed malignancy among women. Long non-coding RNAs (lncRNAs) can be transferred by extracellular vesicles (EVs) to participate in BC progression. This study demonstrated that SNHG16 expression was significantly increased in BC tissues and cells. Overexpression of SNHG16 promoted the migration, invasion, and epithelial-mesenchymal transition (EMT) of BC cells. SNHG16 was carried by EVs. Bioinformatics analysis predicted that SNHG16 regulated PPAPDC1A expression by sponging miR-892b, which was confirmed by RNA-fluorescence in situ hybridization (FISH), RT-qPCR, dual-luciferase gene reporter assay, and RNA immunoprecipitation (RIP). MDA-MB-157 and HS578T cells were transfected with pcDNA3.1-SNHG16, miR-892b-mimic, or si-PPAPDC1A for functional rescue experiments in vitro, and the cells were treated with MDA-MB-231 cell-derived EVs. The results confirmed that enhanced miR-892b expression partially eliminated the increase of migration, invasion, and EMT of BC cells mediated by SNHG16 or EVs. The lung metastasis model in nude mice was established by injecting HS578T cells via tail vein. The results showed that si-SNHG16 reduced the metastatic nodules and decreased the vimentin expression. In conclusion, EVs derived from BC cells transferred SNHG16 via the miR-892b/PPAPDC1A axis, thus promoting EMT, migration, and invasion of BC.
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