期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.709018
关键词
dermatan sulfate (DS); chondroitin sulfate (CS); glycosaminoglycan (GAG); GlycoMaple; heparan sulfate (HS); hyaluronan (HA); keratan sulfate (KS); proteoglycan (PG)
资金
- National Natural Science Foundation of China [32071278, 31770853]
- Program of Introducing Talents of Discipline to Universities [111-2-06]
- International Joint Research Laboratory for Investigation of Glycoprotein Biosynthesis at Jiangnan University
- Japan Society for the Promotion of Science [19K07054]
- Grants-in-Aid for Scientific Research [19K07054] Funding Source: KAKEN
The study developed a comprehensive glycosylation mapping tool, GlycoMaple, and analyzed the expression levels of GAG biosynthetic genes in various human tissues and tumor tissues using this tool.
Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans. More than 50 gene products are involved in the biosynthesis of GAGs. We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles. Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues. In brain and pancreatic tumors, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be upregulated. In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature. In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively. These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
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