期刊
JOURNAL OF EXTRACELLULAR VESICLES
卷 10, 期 10, 页码 -出版社
WILEY
DOI: 10.1002/jev2.12134
关键词
extracellular vesicles; healthy volunteers; lung injury; mesenchymal stromal cells; nebulization
类别
资金
- National Natural Science Foundation of China [81570066, 81630001, 81900072]
- Shanghai Shenkang Hospital Development Center Clinical Science and Technology Innovation Project
MSC-EVs derived from mesenchymal stromal cells have shown promise as a cell-free therapy source. Nebulized haMSC-EVs improved survival rates and lung inflammation in a murine lung injury model, and were well tolerated in healthy volunteers. These findings suggest that nebulized haMSC-EVs could be a promising therapeutic strategy for lung injury diseases.
Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) turn out to be a promising source of cell-free therapy. Here, we investigated the biodistribution and effect of nebulized human adipose-derived MSC-EVs (haMSC-EVs) in the preclinical lung injury model and explored the safety of nebulized haMSC-EVs in healthy volunteers. DiR-labelled haMSC-EVs were used to explore the distribution of nebulized haMSC-EVs in the murine model. Pseudomonas aeruginosa-induced murine lung injury model was established, and survival rate, as well as WBC counts, histology, IL-6, TNF-alpha and IL-10 levels in bronchoalveolar lavage fluid (BALF) were measured to explore the optimal therapeutic dose of haMSC-EVs through the nebulized route. Twenty-four healthy volunteers were involved and received the haMSC-EVs once, ranging from 2 x 10(8) particles to 16 x 10(8) particles (MEXVT study, NCT04313647). Nebulizing haMSC-EVs improved survival rate to 80% at 96 h in P. aeruginosa-induced murine lung injury model by decreasing lung inflammation and histological severity. All volunteers tolerated the haMSC-EVs nebulization well, and no serious adverse events were observed from starting nebulization to the 7th day after nebulization. These findings suggest that nebulized haMSC-EVs could be a promising therapeutic strategy, offering preliminary evidence to promote the future clinical applications of nebulized haMSC-EVs in lung injury diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据