Comparison of the Genetic Structure of Invasive Bigheaded Carp (Hypophthalmichthys spp.) Populations in Central-European Lacustrine and Riverine Habitats

Simple Summary Bigheaded carps (bighead carp and silver carp) originated in Southeast and East Asia, and their hybrids were stocked for economic reasons to Hungarian natural waters such as Lake Balaton for decades, while the Tisza River was populated by escaped individuals with farm origins. The presence of these alien species and their hybrids in Hungarian natural water bodies may pose significant ecological risks (connected with their phytoplankton and zooplankton consumption). To be able to deal with the ecological risks and to understand the potential of invasiveness of these species, one must have information on the population-level genetic structures of these alien fish stocks. Ten microsatellite DNA markers and one mitochondrial marker were used to address these questions. The results showed that the two stocks are genetically different; the lake population was genetically more diverse and consisted of hybrid and silver carp individuals, while the river population contained only silver carps. The mitochondrial sequences found in the two populations originated from the Yangtze River. Based on the different genetic structures of the stocks, one can assume that bigheaded carps do not reproduce in Lake Balaton, while the Tisza River stock represents significant reproductive potential and may become invasive in this river. Bigheaded carps (bighead carp, Hypophthalmichthys molitrix, and silver carp, Hypophthalmichthys nobilis) and their hybrids play an important ecological and economic role in their original habitat, while their introduced stocks may pose serious ecological risks. To address questions about the persistence and invasiveness of these fish, we need to better understand their population structures. The genetic structures of bigheaded carp populations inhabiting Lake Balaton and the Tisza River were examined with ten microsatellite markers and a mitochondrial DNA marker (COI). The Lake Balaton stock showed higher genetic diversity compared with the Tisza River stock. Based on hierarchical clustering, the Tisza population was characterized only by only silver carps, while the Balaton stock included hybrid and silver carp individuals. All COI haplotypes originated from the Yangtze River. Based on the high genomic and mitochondrial diversity, along with the significant deviation from H-W equilibrium and the lack of evidence of bottleneck effect, it can be assumed that bigheaded carps do not reproduce in Lake Balaton. The present stock in Balaton may have originated from repeated introductions and escapes from the surrounding fishponds. The Tisza stock consists solely of silver carp individuals. This stock appears to have significant reproductive potential and may become invasive if environmental factors change due to climate change.

Automated summary

The genetic structures of bigheaded carp populations in Lake Balaton and the Tisza River in Hungary differ, with the lake population being more genetically diverse with hybrid and silver carp individuals, while the river population consists only of silver carps. The mitochondrial sequences found in both populations originated from the Yangtze River, suggesting potential invasive behavior in the river population.