A Study on the Pathological Effects of Trypanorhyncha Cestodes in Dusky Groupers Epinephelus marginatus from the Canary Islands

Simple Summary Trypanorhyncha are common parasites of marine fish. Despite numerous studies detailing their biology, knowledge on the effects caused by these parasites in fish tissues is still limited. Dusky groupers are keystone species, necessary for the preservation of several marine ecosystems. Considering their vulnerable state of conservation and the efforts being made to culture them, identification of the effects caused by Trypanorhyncha is vital. Here, we have assessed the prevalence of Trypanorhyncha in dusky groupers from the Canary Islands and the associated pathological changes. Of the 28 fish examined, 27 presented trypanorhynch larvae. Macroscopically, in the abdominal cavity, there were numerous larvae-filled cysts and nodules embedded in abundant fibrosis, hindering the separation of the organs. Microscopically, in the peritoneum, stomach and intestine, there were numerous degenerated parasitic cysts and extensive deposition of fibrous connective tissue with minimal inflammatory responses. This study shows that Trypanorhyncha are common parasites of adult dusky groupers from the Canary Islands. Even though the immune system appears to isolate and eliminate the parasites, extensive fibrosis may have a detrimental impact on fish health when adjacent organs are compressed and their functions impaired. Trypanorhyncha are cestodes commonly infecting marine fish. Numerous studies have detailed the biology of Trypanorhyncha species, but information on the pathological changes produced by these parasites is limited. Dusky groupers are keystone species necessary for the preservation of several marine ecosystems. Considering their vulnerable state of conservation and the efforts being made to culture them, identification of the effects caused by Trypanorhyncha is vital. Here, we aimed to determine the prevalence and pathological changes produced by Trypanorhyncha in dusky groupers from the Canary Islands. The prevalence of trypanorhynch plerocerci was 96%. Grossly, in the abdominal cavity, there were numerous larvae-filled cysts and nodules. These were embedded in abundant fibrosis, producing visceral adhesions. Histologically, affecting the peritoneum, stomach, and intestine there were numerous degenerated encysted plerocerci and extensive deposition of mature connective tissue. These findings indicate that Trypanorhyncha is highly prevalent in adult dusky groupers from the Canary Islands, producing a progressive and chronic response. Furthermore, fish immune system appears to attempt to eliminate the parasites through fibrous encapsulation. Nonetheless, extensive fibrosis may have a detrimental impact on fish health when adjacent cells or tissues are compressed and their functions impaired.

Automated summary

This study found that Trypanorhyncha is a common parasite infecting adult dusky groupers in the Canary Islands, causing cysts and nodules in the abdominal cavity and fibrosis in the peritoneum, stomach, and intestine. While the fish immune system appears to eliminate the parasites, extensive fibrosis may negatively impact fish health when adjacent organs are compressed and their functions impaired.