期刊
PROCESSES
卷 9, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/pr9071196
关键词
butanol; ABE; IBE; core genome; accessory genome; pan genome
Clostridium beijerinckii, a widely studied bacterium, was analyzed for its genome diversity and evolution, revealing misidentification of certain strains and defining the pangenome of the species. The core genome contains genes for basic metabolism, while the accessory genome affects final phenotype, showing great diversity within the species with potential for biotechnological applications.
Clostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity of the whole species has not been studied, and it has only been analyzed in part for a missing genome of the type strain. Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791(T), composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. Additionally, we filtered possibly misassembled genomes, and we used the remaining 237 high-quality genomes to define the pangenome of the whole species. By its functional annotation, we showed that the core genome contains genes responsible for basic metabolism, while the accessory genome has genes affecting final phenotype that may vary among different strains. We used the core genome to reconstruct the phylogeny of the species and showed its great diversity, which complicates the identification of particular strains, yet hides possibilities to reveal hitherto unreported phenotypic features and processes utilizable in biotechnology.
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