4.6 Article

Cooperation Between Cancer and Fibroblasts in Vascular Mimicry and N2-Type Neutrophil Recruitment via Notch2-Jagged1 Interaction in Lung Cancer

期刊

FRONTIERS IN ONCOLOGY
卷 11, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2021.696931

关键词

cancer-associated fibroblasts; Jagged1; lung cancer; Notch2; vascular mimicry

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资金

  1. Ministry of Science and Technology [MOST 110-2314-B-037 -124 -MY3, 109-2314-B-037-091, 109-2314-B-037-126-MY2, 108-2320-B-037-024-MY3, 108-2314-B-037-094-, 108-2314-B-037-095-, 107-2314-B-037-107-MY3]
  2. Kaohsiung Medical University [KMU-DK 109002, KMU-DK108003, KMU-DK108008, KMU-TC108A03-5]
  3. Kaohsiung Medical University Hospital [KMUH-109-9R12, KMUH-109-9M13]

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This study verified the role of cancer-associated fibroblasts (CAFs) in inducing lung cancer to create vascular-like networks, facilitating tumor development and metastasis. Disruption of the connections between cancer cells and CAFs can enhance the anti-tumor effect of anti-vascular endothelial growth factor antibodies.
Background Angiogenesis is required for tumor development and metastasis, which is a major part in a pro-tumor microenvironment. Vascular mimicry (VM) is a process in which cancer cells, rather than endothelia, create an alternative perfusion system to support the tumor progression. & nbsp; Objectives To validate the role of VM and to develop a strategy to inhibit angiogenesis in lung cancer. & nbsp; Methods In this study, we utilized lung cancer samples to verify the existence of VM and conducted several experimental methods to elucidate the molecular pathways. & nbsp; Results H1299 and CL1-0 lung cancer cells were unable to form capillary-like structures. VM formation was induced by cancer-associated fibroblast (CAFs) in both in vitro and in vivo experiments. Notch2-Jagged1 cell-cell contact between cancer cells and CAFs contributes to the formation of VM networks, supported by Notch intracellular domain (NICD) 2 nuclear translocation and N2ICD target gene upregulated in lung cancer cells mixed with CAFs. The polarization of tumor-promoting N2-type neutrophil was increased by VM networks consisting of CAF and cancer cells. The intravasation of cancer cells and N2-type neutrophils were increased because of the loose junctions of VM. Disruption of cancer cell-CAF connections by a gamma-secretase inhibitor enforced the anticancer effect of anti-vascular endothelial growth factor antibodies in a mouse model. & nbsp; Conclusion This study provides the first evidence that CAFs induce lung cancer to create vascular-like networks. These findings suggest a therapeutic opportunity for improving antiangiogenesis therapy in lung cancer.

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