期刊
CELLS
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/cells10092442
关键词
cleaner fish; lumpfish; respiration; epithelial; endothelial; virus; animal welfare; 3R; medical delousing; marine fish
类别
资金
- Norwegian Veterinary Institute internal strategic initiative BIO-Direct: Biomarkers and Bioassays for Veterinary Research and Diagnostics
The establishment of the first Lumpfish Gill cell line (LG-1) provides valuable insights into the biology of lumpfish, with the cells originating from an epithelial or endothelial cell type. LG-1 can serve as an important in vitro research tool for studying gill cell function and host-pathogen interactions in lumpfish.
The use of lumpfish (Cyclopterus lumpus) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill cell line (LG-1). LG-1 are adherent, homogenous and have a flat, stretched-out and almost transparent appearance. Transmission electron microscopy revealed cellular protrusions and desmosome-like structures that, together with their ability to generate a transcellular epithelial/endothelial resistance, suggest an epithelial or endothelial cell type. Furthermore, the cells exert Cytochrome P450 1A activity. LG-1 supported the propagation of several viruses that may lead to severe infectious diseases with high mortalities in fish farming, including viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). Altogether, our data indicate that the LG-1 cell line originates from an epithelial or endothelial cell type and will be a valuable in vitro research tool to study gill cell function as well as host-pathogen interactions in lumpfish.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据