期刊
CELLS
卷 10, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/cells10061354
关键词
transcriptionally active chromatin; compartment A and B; phase separation; histone modifications; chromatin-modifying enzymes
类别
资金
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2017-05927]
- CancerCare Manitoba Foundation [761020318]
The chicken erythrocyte model system is valuable for studying chromatin structure and function, with unique features allowing the separation of transcriptionally active chromatin and silenced chromatin. Recent studies have mapped transcriptionally active chromosomal domains in the chicken erythrocyte genome and reported detailed landscapes of histone modifications in relation to chromatin structural features.
The chicken erythrocyte model system has been valuable to the study of chromatin structure and function, specifically for genes involved in oxygen transport and the innate immune response. Several seminal features of transcriptionally active chromatin were discovered in this system. Davie and colleagues capitalized on the unique features of the chicken erythrocyte to separate and isolate transcriptionally active chromatin and silenced chromatin, using a powerful native fractionation procedure. Histone modifications, histone variants, atypical nucleosomes (U-shaped nucleosomes) and other chromatin structural features (open chromatin) were identified in these studies. More recently, the transcriptionally active chromosomal domains in the chicken erythrocyte genome were mapped by combining this chromatin fractionation method with next-generation DNA and RNA sequencing. The landscape of histone modifications relative to chromatin structural features in the chicken erythrocyte genome was reported in detail, including the first ever mapping of histone H4 asymmetrically dimethylated at Arg 3 (H4R3me2a) and histone H3 symmetrically dimethylated at Arg 2 (H3R2me2s), which are products of protein arginine methyltransferases (PRMTs) 1 and 5, respectively. PRMT1 is important in the establishment and maintenance of chicken erythrocyte transcriptionally active chromatin.
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