4.6 Article

N-Glycomic Analysis of the Cell Shows Specific Effects of Glycosyl Transferase Inhibitors

期刊

CELLS
卷 10, 期 9, 页码 -

出版社

MDPI
DOI: 10.3390/cells10092318

关键词

mass spectrometry; N-glycan; glycosyltransferase

资金

  1. General Medicine of the National Institutes of Health [R01GM049077]

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Glycomic profiling methods were used to study the effect of metabolic inhibitors on glycan production, with kifunensine being the most effective in converting nearly 95% of glycans to high mannose types. Other inhibitors also had an impact on glycan structures. Future research could utilize these inhibitors to investigate cellular behavior associated with alterations in glycosylation in various biological systems.
Glycomic profiling methods were used to determine the effect of metabolic inhibitors on glycan production. These inhibitors are commonly used to alter the cell surface glycosylation. However, structural analysis of the released glycans has been limited. In this research, the cell membranes were enriched and the glycans were released to obtain the N-glycans of the glycocalyx. Glycomic analysis using liquid chromatography-mass spectrometry (LC-MS) with a PGC chip column was used to profile the structures in the cell membrane. Glycans of untreated cells were compared to glycans of cells treated with inhibitors, including kifunensine, which inhibits the formation of complex- and hybrid-type structures, 2,4,7,8,9-Penta-O-acetyl-N-acetyl-3-fluoro-b-d-neuraminic acid methyl ester for sialylated glycans, 2-deoxy-2-fluorofucose, and 6-alkynyl fucose for fucosylated glycans. Kifunensine was the most effective, converting nearly 95% of glycans to high mannose types. The compound 6-alkynyl fucose inhibited some fucosylation but also incorporated into the glycan structure. Proteomic analysis of the enriched membrane for the four inhibitors showed only small changes in the proteome accompanied by large changes in the N-glycome for Caco-2. Future works may use these inhibitors to study the cellular behavior associated with the alteration of glycosylation in various biological systems, e.g., viral and bacterial infection, drug binding, and cell-cell interactions.

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