4.6 Article

Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease

期刊

出版社

BMC
DOI: 10.1186/s40478-021-01216-4

关键词

Alzheimer; Amyloid; Astrocyte; Inflammation; Lipid mediators; Microglia; Phospholipids; Resolution

资金

  1. Karolinska Institute
  2. Swedish Research Council [22743, 22744]
  3. Swedish Alzheimer Foundation
  4. Karolinska Institutet research funds
  5. Ahlen-Stiftelsen
  6. Stiftelsen for Gamla Tjanarinnor
  7. Gun och Bertil Stohnes Stiftelse
  8. Hallstens forskningsstiftelse
  9. Torsten Soderbergs Stiftelse
  10. EENT Foundation of New Orleans

向作者/读者索取更多资源

This study investigated lipid and cytokine profiles, glial activation, and inflammatory proteins in an APP knock-in AD mouse model. It found age-specific changes in the brain lipidome, with increased pro-inflammatory and pro-resolving lipid mediators only in older age groups despite early Aβ pathology. Additionally, alterations in phospholipids signaled early pathological changes in membrane composition.
Sustained brain chronic inflammation in Alzheimer's disease (AD) includes glial cell activation, an increase in cytokines and chemokines, and lipid mediators (LMs), concomitant with decreased pro-homeostatic mediators. The inflammatory response at the onset of pathology engages activation of pro-resolving, pro-homeostatic LMs followed by a gradual decrease. We used an APP knock-in (App KI) AD mouse that accumulates beta -amyloid (A beta) and presents cognitive deficits (at 2 and 6 months of age, respectively) to investigate LMs, their precursors, biosynthetic enzymes and receptors, glial activation, and inflammatory proteins in the cerebral cortex and hippocampus at 2-, 4-, 8- and 18-month-old in comparison with wild-type (WT) mice. We used LC-mass-spectrometry and MALDI molecular imaging to analyze LMs and phospholipids, and immunochemistry for proteins. Our results revealed an age-specific lipid and cytokine profile, and glial activation in the App KI mice. Despite an early onset of A beta pathology, pro-inflammatory and pro-resolving LMs were prominently increased only in the oldest age group. Furthermore, the LM biosynthetic enzymes increased, and their receptor expression decreased in the aged App KI mice. Arachidonic acid (AA)-containing phospholipid molecular species were elevated, correlating with decreased cPLA2 activity. MALDI molecular imaging depicted differential distribution of phospholipids according to genotype in hippocampal layers. Brain histology disclosed increased microglia proliferation starting from young age in the App KI mice, while astrocyte numbers were enhanced in older ages. Our results demonstrate that the brain lipidome is modified preferentially during aging as compared to amyloid pathology in the model studied here. However, alterations in phospholipids signal early pathological changes in membrane composition.

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