期刊
JOURNAL OF CLINICAL MEDICINE
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/jcm10112309
关键词
mass spectrometry; proteomics; bioinformatics; vitreous; proliferative diabetic retinopathy
资金
- Coulter Translational Research Partnership Program, Taubman Institute
- Bennet and Inez Chotiner Early Career Assistant Professorship
- Research to Prevent Blindness
- JDRF Center of Excellence at the University of Michigan
Vitreous fluid has emerged as a valuable medium for studying retinal diseases, with proteomic analysis providing insights into proliferative diabetic retinopathy. Despite the lack of standardized methodology, there have been numerous studies utilizing mass spectrometry for vitreous proteomic analysis. Moving forward, addressing common pitfalls and proposing next steps is crucial for advancing this field.
Vitreous fluid is becoming an increasingly popular medium for the study of retinal disease. Numerous studies have demonstrated that proteomic analysis of the vitreous from patients with proliferative diabetic retinopathy yields valuable molecular information regarding known and novel proteins and pathways involved in this disease. However, there is no standardized methodology for vitreous proteomic studies. Here, we share a suggested protocol for such studies and outline the various experimental and analytic methods that are currently available. We also review prior mass spectrometry-based proteomic studies of the vitreous from patients with proliferative diabetic retinopathy, discuss common pitfalls of these studies, and propose next steps for moving the field forward.
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