期刊
ACTA PHARMACEUTICA SINICA B
卷 12, 期 2, 页码 821-837出版社
INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
DOI: 10.1016/j.apsb.2021.06.009
关键词
Acidic tumor microenvironment; miR-7-5p; TGF-beta 2; Metastasis; Lung cancer; pH; Invasion
资金
- National Natural Science Foundation of China [81874367, 82074019]
- Guangdong Key Laboratory for Translational Cancer research of Chinese Medicine (China) [2018B030322011]
- Natural Science Foundation for Distinguished Young Scholars of Guangdong Province, China [2017A030306033]
- Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2016, China)
- Project of Educational Commission of Guangdong Province of China [2016KTSCX012]
- Pearl River Nova Program of Guangzhou, China [201710010108]
Acidosis is a harsh tumor microenvironment that promotes lung cancer metastasis by inhibiting miR-7-5p expression and increasing TGF-beta 2 expression. The study identifies miR-7-5p as a novel biomarker and therapeutic target for lung cancer treatment.
Acidosis, regardless of hypoxia involvement, is recognized as a chronic and harsh tumor microenvironment (TME) that educates malignant cells to thrive and metastasize. Although overwhelming evidence supports an acidic environment as a driver or ubiquitous hallmark of cancer progression, the unrevealed core mechanisms underlying the direct effect of acidification on tumorigenesis have hindered the discovery of novel therapeutic targets and clinical therapy. Here, chemical-induced and transgenic mouse models for colon, liver and lung cancer were established, respectively. miR-7 and TGF-beta 2 expressions were examined in clinical tissues (n = 184). RNA-seq, miRNA-seq, proteomics, biosynthesis analyses and functional studies were performed to validate the mechanisms involved in the acidic TME-induced lung cancer metastasis. Our data show that lung cancer is sensitive to the increased acidification of TME, and acidic TME-induced lung cancer metastasis via inhibition of miR7-5p. TGF-beta 2 is a direct target of miR-7-5p. The reduced expression of miR-7-5p subsequently increases the expression of TGF-beta 2 which enhances the metastatic potential of the lung cancer. Indeed, overexpression of miR-7-5p reduces the acidic pH-enhanced lung cancer metastasis. Furthermore, the human lung tumor samples also show a reduced miR-7-5p expression but an elevated level of activated TGF-132; the expressions of both miR-7-5p and TGF-beta 2 are correlated with patients' survival. We are the first to identify the role of the miR-7/TGF-beta 2 axis in acidic pH-enhanced lung cancer metastasis. Our study not only delineates how acidification directly affects tumorigenesis, but also suggests miR-7 is a novel reliable biomarker for acidic TME and a novel therapeutic target for non-small cell lung cancer (NSCLC) treatment. Our study opens an avenue to explore the pH-sensitive subcellular components as novel therapeutic targets for cancer treatment. (C) 2022 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
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