期刊
SCIENCE ADVANCES
卷 7, 期 24, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abf8210
关键词
-
资金
- CREST program of the Japan Science and Technology Agency (JST) [JPMJCR13W6, JPMJCR1922]
- Japan Society for the Promotion of Science [17KT0020, 19H05670, 20J40280]
- Grants-in-Aid for Scientific Research [19H05670, 20J40280, 17KT0020] Funding Source: KAKEN
The study revealed how gene regulatory networks control gene expression dynamics in ascidian embryos, including the regulatory logic and core mechanism at the 32-cell stage, offering important insights into the operating principles of gene regulatory networks.
In animal embryos, gene regulatory networks control the dynamics of gene expression in cells and coordinate such dynamics among cells. In ascidian embryos, gene expression dynamics have been dissected at the single-cell resolution. Here, we revealed mathematical functions that represent the regulatory logics of all regulatory genes expressed at the 32-cell stage when the germ layers are largely specified. These functions collectively explain the entire mechanism by which gene expression dynamics are controlled coordinately in early embryos. We found that regulatory functions for genes expressed in each of the specific lineages contain a common core regulatory mechanism. Last, we showed that the expression of the regulatory genes became reproducible by calculation and controllable by experimental manipulations. Thus, these regulatory functions represent an architectural design for the germ layer specification of this chordate and provide a platform for simulations and experiments to understand the operating principles of gene regulatory networks.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据