期刊
SCIENCE ADVANCES
卷 7, 期 36, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abg4585
关键词
-
资金
- National Natural Science Foundation of China [21808111]
- National Natural Science Foundation of Jiangsu Province [BK20170829, BK20170037]
A highly efficient biocatalyst was developed to selectively produce gallate from lignin components, achieving high yields of 0.407 and 0.630 g of gallate per gram of lignin when using different lignin substrates. This biocatalyst shows promise for simplifying and improving the lignin valorization process.
Converting lignin components into a single product is a promising way to upgrade lignin. Here, an efficient biocatalyst was developed to selectively produce gallate from lignin components by integrating three main reactions: hydroxylation, O-demethylation, and aryl side-chain oxidation. A rationally designed hydroxylase system was first introduced into a gallate biodegradation pathway-blocked Rhodococcus opacus mutant so that gallate accumulated from protocatechuate and compounds in its upper pathways. Native and heterologous O-demethylation systems were then used, leading to multiple lignin-derived methoxy aromatics being converted to gallate. Furthermore, an aryl side-chain oxidase was engaged to broaden the substrate spectrum. Consequently, the developed biocatalyst showed that gallate yields as high as 0.407 and 0.630 g of gallate per gram of lignin when alkaline-pretreated lignin and base-depolymerized ammonia fiber explosion lignin were applied as substrates, respectively. These results suggested that this rationally developed biocatalyst enabled the lignin valorization process to be simple and efficient.
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