期刊
ACS SENSORS
卷 6, 期 6, 页码 2141-2146出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.1c00961
关键词
pH switching; ratiometric probe; lysosome targeting; live-cell imaging; cyanine dye
资金
- DST-SERB [ECR/2017/001405]
In this study, a pH-activatable fluorescent dye was designed and synthesized, which can switch from visible to near-infrared spectrum under acidic conditions and be controlled by pH. The properties of this fluorescent dye were monitored using NMR, UV/vis, and fluorescence spectroscopies. The dye showed promising performance in live cell lysosomal imaging and has potential applications.
Here, we have designed and synthesized acidic pH-activatable visible to NIR switchable ratiometric pH-sensitive fluorescent dye. The design consists of a cell-permeable organic probe containing a lysosome targeting morpholine functionality and an acidic pH-activatable oxazolidine moiety. The visible closed oxazolidine form (lambda(abs) 418 nm) can be switched to the highly conjugated NIR Cy-7 form (lambda(abs) 780 nm) through ring opening of the oxazolidine moiety at acidic pH. This switching of the ratiometric fluorescent probe is highly reversible and can be controlled by pH. NMR, UV/vis, and fluorescence spectroscopies allowed monitoring of pH switching behavior of the probe. This bioresponsive in situ acidic organelle activatable fluorophore showed reversible pH-switchable ratiometric optical properties, high photostability, huge bathochromic emission shift of 320 nm from basic to acidic pH, off-to-on narrow NIR absorption and emission bands with enhanced molar extinction coefficient at lysosomal pH, good quantum yield, low cytotoxicity, and targeted imaging ability of live cell lysosomes with ideal pK(a). The report demonstrated ratiometric imaging with improved specificity of the acidic lysosome while minimizing signals at the NIR region from nontargeted neutral or basic organelles in human carcinoma HeLa and A549 as well as rat healthy H9c2(2-1) live cells, which is monitored by confocal laser scanning microscopy.
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