期刊
FOOD BIOSCIENCE
卷 42, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fbio.2021.101198
关键词
Electron beam irradiation; Bacterial community; Portunus trituberculatus; 16S rRNA
资金
- National Key R&D Program of China [2020YFD0900903]
- Research on the key technology of fresh agricul-tural products storage and transportation logistics-the research and industrialization demonstration of the first-line fresh-keeping and cold chain logistics technology of sea-caught shrimp [2019C02086]
- Marine Economy of the State Oceanic Administration [NBHY-2017-S2]
This study used high-throughput sequencing technologies to investigate the impact of different doses of electron beam irradiation (EBI) on the microbial community structure of Portunus trituberculatus, finding significant changes with increasing radiation doses. The richness and diversity of microorganisms showed variation with the radiation dose, with a minimum EBI dose of 6 kGy identified. The study provides a theoretical basis for crab preservation.
Electron beam irradiation (EBI) can effectively kill microorganisms that cause food decay and thus, prolong the shelf life of food. However, different doses of EBI on the impact of Portunus trituberculatus microbial community structure remain to be seen. This study adopted high-throughput sequencing technologies to compare the effect of different doses of EBI on the microbial community structure of the swimming crab and found that the crab microbial community structure was changed significantly upon treatment with different doses of EBI. We combined alpha and beta diversity analysis, relative abundance analysis of species, and linear discriminant analysis effect size analysis, and our results focused on Psychrilyobacter, Clostridium, Vagococcus, Psychrobacter, and Carnobacterium. In addition, the richness and diversity of microorganisms initially decreased and then increased with the increasing of radiation dose. The results showed that the minimum dose of EBI was 6 kGy. This study provides a theoretical basis for the preservation of crabs.
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