Ganoderma amboinense polysaccharide prevents obesity by regulating gut microbiota in high-fat-diet mice

Obesity has caused serious economic and health problems in the world. Gut microbiota is a complex ecosystem and plays an important role in preventing obesity. The purpose of this study was to investigate the effects of Ganoderma amboinense (G. amboinense) polysaccharide (GAP) on obesity-related characteristics in high-fat-diet (HFD) mice, and to determine whether GAP treatment can regulate gut microbiota in HFD-mice. GAP was extracted from G. amboinense by hot water extracting with further separation and purification. Male C57BL/6 mice were fed with HFD and supplemented with GAP for 8 weeks. The results showed that GAP had a regulatory effect on the gut microbiota of HFD-mice, the abundance of 3 genera and multiple keys signaling pathways were regulated. And GAP treatment effectively prevented weight gain and fat accumulation, improved glucose tolerance, and reduced serum and liver lipid concentrations and inflammation in HFD-mice.

Automated summary

GAP can regulate the gut microbiota of HFD-mice and effectively prevent weight gain and fat accumulation, improve glucose tolerance, reduce serum and liver lipid concentrations, and inflammation.