Double molecular recognition ligands modified CPDS/CMCS/Fe3O4-rGO hybrid with enhanced enrichment capacity for glycoproteins at neutral environment

In order to achieve specific enrichment of target glycoproteins, double molecular recognition ligands functionalized surface solid-phase adsorption materials that can play a role under physiological pH conditions, have obvious advantages. In this work, a novel strategy was proposed for the synthesis of double molecular recognition functionalized magnetic graphene nanocomposites. Horseradish peroxidase was selected as the template proteins for evaluating the selectivity and binding capacity of the adsorption material. The bimolecular recognition was realized by introducing 4-carboxyphenylboronic acid (CPBA) and Suberic acid bis(3-sulfo-N-hydroxysuccinimide ester) sodium salt (Sulfo-DSS) as functional ligands. The abundant oxygen-containing groups in the complex (abbreviated as CPDS/CMCS/Fe3O4-rGO) were beneficial to reduce the pKa value of CPBA. Finally, CPDS/CMCS/Fe3O4-rGO realized the selective adsorption of glycoproteins in the neutral environment (pH 7.0). The resultant CPDS/CMCS/Fe3O4-rGO exhibited a high adsorption capacity of 1280.6 mg g(-1) and excellent specificity toward glycoproteins compared to non-glycoproteins. Moreover, CPDS/CMCS/Fe3O4-rGO with strong magnetic responses (59.29 emu.g(-1)), could achieve rapid aggregation and separation under the external magnetic field. The results demonstrated the great potential of CPDS/CMCS/Fe3O4-rGO composites to separate and enrich glycoproteins from the complex biological sample for the glycoproteins analysis. (C) 2021 The Author(s). Published by Elsevier B.V.

Automated summary

A novel strategy for the synthesis of double molecular recognition functionalized magnetic graphene nanocomposites was proposed to selectively adsorb glycoproteins in a neutral environment. The composite exhibited high adsorption capacity and rapid separation, with excellent specificity towards glycoproteins. The results suggest great potential for separating and enriching glycoproteins from complex biological samples for analysis.