4.6 Article

High-Throughput Sequencing-Based Identification of miRNAs and Their Target mRNAs in Wheat Variety Qing Mai 6 Under Salt Stress Condition

期刊

FRONTIERS IN GENETICS
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2021.724527

关键词

wheat; salt stress; transcriptome; microRNA; target gene; expression verification

资金

  1. Foundation of Research and Application of Whole Genome Selection in Wheat [2019LZGC016]
  2. Wheat Innovation Team of Modern Agricultural Production Systems in Shandong Province [SDAIT01-05]
  3. High-Level Talents Project of Qingdao Agricultural University [663/1119013]
  4. National Natural Science Foundation of China [31601231]

向作者/读者索取更多资源

This study identified salt-tolerance-related miRNAs and their target mRNAs in a salt-tolerant wheat variety, QM6, under salt stress conditions. It was found that QM6 enhances salt tolerance by increasing the expression of genes related to stress resistance, antioxidation, nutrient absorption, and lipid metabolism balance, which are regulated by the identified miRNAs. This data provides a theoretical basis for future research on miRNAs and novel genes related to salt tolerance in wheat for the development of genetically improved salt-tolerant wheat varieties.
Soil salinization is one of the major abiotic stresses that adversely affect the yield and quality of crops such as wheat, a leading cereal crop worldwide. Excavating the salt-tolerant genes and exploring the salt tolerance mechanism can help breeding salt-tolerant wheat varieties. Thus, it is essential to identify salt-tolerant wheat germplasm resources. In this study, we carried out a salt stress experiment using Qing Mai 6 (QM6), a salt-tolerant wheat variety, and sequenced the miRNAs and mRNAs. The differentially expressed miRNAs and mRNAs in salt stress conditions were compared with the control. As results, a total of eight salt-tolerance-related miRNAs and their corresponding 11 target mRNAs were identified. Further analysis revealed that QM6 enhances salt tolerance through increasing the expression level of genes related to stress resistance, antioxidation, nutrient absorption, and lipid metabolism balance, and the expression of these genes was regulated by the identified miRNAs. The resulting data provides a theoretical basis for future research studies on miRNAs and novel genes related to salt tolerance in wheat in order to develop genetically improved salt-tolerant wheat varieties.

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