期刊
FRONTIERS IN GENETICS
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2021.698163
关键词
predicted deleterious SNPs; MYBPH; abdominal fat; chicken; meat-type
资金
- Sao Paulo Research Foundation (FAPESP) [14/087040]
- Brazilian Agricultural Research Corporation -Embrapa [01.11.07.002.04.02]
- National Council of Scientific and Technological Development (CNPq) from the Brazilian Government [481755/2007-1]
- FAPESP [14/21380-9, 2016/13589-0, 16/00569-1]
- program Science without Borders from CNPq [370620/2013-5]
- CNPq
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [16/00569-1] Funding Source: FAPESP
The study aimed to identify predicted deleterious mutations affecting carcass traits in meat-type chickens and provided evidence that the c.482C > T SNP in the MYBPH gene may be a potential causal mutation for fat deposition.
Chicken is an important source of protein for human nutrition and a model system for growth and developmental biology. Although the genetic architecture of quantitative traits in meat-type chickens has been the subject of ongoing investigation, the identification of mutations associated with carcass traits of economic interest remains challenging. Therefore, our aim was to identify predicted deleterious mutation, which potentially affects protein function, and test if they were associated with carcass traits in chickens. For that, we performed a genome-wide association analysis (GWAS) for breast, thigh and drumstick traits in meat-type chickens and detected 19 unique quantitative trait loci (QTL). We then used: (1) the identified windows; (2) QTL for abdominal fat detected in a previous study with the same population and (3) previously obtained whole genome sequence data, to identify 18 predicted deleterious single nucleotide polymorphisms (SNPs) in those QTL for further association with breast, thigh, drumstick and abdominal fat traits. Using the additive model, a predicted deleterious SNP c.482C > T (SIFT score of 0.4) was associated (p-value < 0.05) with abdominal fat weight and percentage. This SNP is in the second exon of the MYBPH gene, and its allele frequency deviates from Hardy-Weinberg equilibrium. In conclusion, our study provides evidence that the c.482C > T SNP in the MYBPH gene is a putative causal mutation for fat deposition in meat-type chickens.
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