Localization of RNA Pol II CTD (S5) and Transcriptome Analysis of Testis in Diploid and Tetraploid Hybrids of Red Crucian Carp (♀) x Common Carp (♂)

Polyploidy occurs naturally in fish; however, the appearance of these species is an occasional and gradual process, which makes it difficult to trace the changes in phenotypes, genotypes, and regulation of gene expression. The allotetraploid hybrids (4nAT) of red crucian carp (RCC; female) x common carp (CC; male) generated from interspecies crossing are a good model to investigate the initial changes after allopolyploidization. In the present study, we focused on the changes in the active sites of the testicular transcriptome of the allotetraploid by localization of RNA Pol II CTD YSPTSPS (phospho S5) using immunofluorescence and RNA-seq data via bioinformatic analysis. The results showed that there was no significant difference in signal counts of the RNA Pol II CTD (S5) between the different types of fish at the same stages, including RCC, CC, 2nF(1), and 4nAT, which means that the number of transcriptionally active sites on germ cell chromosomes was not affected by the increase in chromosome number. Similarly, RNA-seq analysis indicated that in the levels of chromosomes and 10-kb regions in the genome, there were no significant changes in the highly active sites in RCC, 2nF(1), and 4nAT. These findings suggest that at the beginning of tetraploid origin, the active transcriptome site of 4nAT in the testis was conserved in the regions of the genome compared to that in RCC and 2nF(1). In conclusion, 4nAT shared a similar gene expression model in the regions of the genome with RCC and 2nF(1) with significantly different expression levels.

Automated summary

The study focused on changes in active sites of the testicular transcriptome of allotetraploid fish by analyzing RNA Pol II CTD YSPTSPS (phospho S5) and RNA-seq data. Results showed no significant difference in transcriptionally active sites between different types of fish, suggesting chromosome number increase did not affect transcriptionally active sites. RNA-seq analysis further indicated no significant changes in highly active sites in the genome regions, implying conservation of active transcriptome sites in allotetraploid fish compared to diploid fish.