期刊
FRONTIERS IN CHEMISTRY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2021.688442
关键词
magnetic relaxation switching; microfluidic chip; alpha-fetoprotein; immunosensor; nanoprobe
资金
- National Natural Science Foundation of China [52075138]
- Natural Science Foundation of Jiangsu [BK20190872]
- China Scholarship Council [201808140212]
A novel microfluidic chip-based magnetic relaxation switching (MRS) immunosensor using enzyme-mediated nanoparticles was developed to simplify operation and amplify signals, achieving a satisfactory limit of detection for alpha-fetoprotein (AFP) in real samples, outperforming conventional ELISA.
Conventional immunoassay methods have their common defects, such as tedious processing steps and inadequate sensitivity, in detecting whole blood. To overcome the above problems, we report a microfluidic chip-based magnetic relaxation switching (MRS) immunosensor via enzyme-mediated nanoparticles to simplify operation and amplify the signal in detecting whole blood samples. In the silver mirror reaction with catalase (CAT) as the catalyst, H2O2 can effectively control the production of Ag NPs. The amount of Ag NPs formed further affects the degree of aggregation of magnetic nanoparticles (MNPS), which gives rise to the changes of transverse relaxation time (T-2). Both sample addition and reagent reaction are carried out in the microfluidic chip, thereby saving time and reagent consumption. We also successfully apply the sensor to detect alpha-fetoprotein (AFP) in real samples with a satisfied limit of detection (LOD = 0.56 ng/ml), which is superior to the conventional ELISA.
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