期刊
ACS CENTRAL SCIENCE
卷 7, 期 8, 页码 1368-1380出版社
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.1c00596
关键词
-
资金
- Chicago Immunoengineering Innovation Center of the University of Chicago
- Chicago Biomedical Consortium COVID-19 Response Award [CR002]
- NIH NHLBI [T32-HL007605]
- Canadian Institutes of Health Research [201910MFE-430736-73744]
- University of Chicago Comprehensive Cancer Center (Sigal Fellowship in ImmunoOncology)
- NIH NCI [F30-CA221250]
- NIH NIGMS [T32-GM007281]
- NIH NIAID [HHSN272201400008C, P30CA014599]
- Soft Matter Characterization Facility [CHE-1048528, SCR_019198, SCR_017916]
The study found that decorating multiple copies of the SARS-CoV-2 spike protein receptor-binding domain (RBD) on the surface of polymersomes can promote a potent neutralizing antibody response. Both RBDsurf and RBDencap induce robust T cell immune responses, producing multiple Th1-type cytokines.
The COVID-19 pandemic underscores the need for rapid, safe, and effective vaccines. In contrast to some traditional vaccines, nanoparticle-based subunit vaccines are particularly efficient in trafficking antigens to lymph nodes, where they induce potent immune cell activation. Here, we developed a strategy to decorate the surface of oxidation-sensitive polymersomes with multiple copies of the SARS-CoV-2 spike protein receptor-binding domain (RBD) to mimic the physical form of a virus particle. We evaluated the vaccination efficacy of these surface-decorated polymersomes (RBDsurf) in mice compared to RBD-encapsulated polymersomes (RBDencap) and unformulated RBD (RBDfree), using monophosphoryl-lipid-A-encapsulated polymersomes (MPLA PS) as an adjuvant. While all three groups produced high titers of RBD-specific IgG, only RBDsurf elicited a neutralizing antibody response to SARS-CoV-2 comparable to that of human convalescent plasma. Moreover, RBDsurf was the only group to significantly increase the proportion of RBD-specific germinal center B cells in the vaccination-site draining lymph nodes. Both RBDsurf and RBDencap drove similarly robust CD4(+) and CD8(+) T cell responses that produced multiple Th1-type cytokines. We conclude that a multivalent surface display of spike RBD on polymersomes promotes a potent neutralizing antibody response to SARS-CoV-2, while both antigen formulations promote robust T cell immunity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据