4.7 Article

S-nitrosothiols, and other products of nitrate metabolism, are increased in multiple human blood compartments following ingestion of beetroot juice

期刊

REDOX BIOLOGY
卷 43, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.redox.2021.101974

关键词

Nitrate; Nitrite; S-nitrosothiols (RSNO); Plasma; Red blood cells (RBCs); Beetroot (BR)

资金

  1. University of Bisha (Saudi Arabia)
  2. Royal Embassy of the Saudi Arabia Cultural Bureau in the UK

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Ingestion of beetroot juice rich in nitrate increases the concentration of RSNO in red blood cells and plasma, potentially playing a role in nitrate metabolism and actions.
Ingested inorganic nitrate (NO3-) has multiple effects in the human body including vasodilation, inhibition of platelet aggregation, and improved skeletal muscle function. The functional effects of oral NO3- involve the in vivo reduction of NO3- to nitrite (NO2-) and thence to nitric oxide (NO). However, the potential involvement of Snitrosothiol (RSNO) formation is unclear. We hypothesised that the RSNO concentration ([RSNO]) in red blood cells (RBCs) and plasma is increased by NO3--rich beetroot juice ingestion. In healthy human volunteers, we tested the effect of dietary supplementation with NO3--rich beetroot juice (BR) or NO3--depleted beetroot juice (placebo; PL) on [RSNO], [NO3-] and [NO2-] in RBCs, whole blood and plasma, as measured by ozone-based chemiluminescence. The median basal [RSNO] in plasma samples (n = 22) was 10 (5-13) nM (interquartile range in brackets). In comparison, the median values for basal [RSNO] in the corresponding RBC preparations (n = 19) and whole blood samples (n = 19) were higher (p < 0.001) than in plasma, being 40 (30-60) nM and 35 (25-80) nM, respectively. The median RBC [RSNO] in a separate cohort of healthy subjects (n = 5) was increased to 110 (93-125) nM after ingesting BR (12.8 mmol NO3-) compared to a corresponding baseline value of 25 (21-31) nM (Mann-Whitney test, p < 0.01). The median plasma [RSNO] in another cohort of healthy subjects (n = 14) was increased almost ten-fold to 104 (58-151) nM after BR supplementation (7 x 6.4 mmol of NO3- over two days, p < 0.01) compared to PL. In conclusion, RBC and plasma [RSNO] are increased by BR ingestion. In addition to NO2-, RSNO may be involved in dietary NO3- metabolism/actions.

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