期刊
REDOX BIOLOGY
卷 43, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.redox.2021.101974
关键词
Nitrate; Nitrite; S-nitrosothiols (RSNO); Plasma; Red blood cells (RBCs); Beetroot (BR)
资金
- University of Bisha (Saudi Arabia)
- Royal Embassy of the Saudi Arabia Cultural Bureau in the UK
Ingestion of beetroot juice rich in nitrate increases the concentration of RSNO in red blood cells and plasma, potentially playing a role in nitrate metabolism and actions.
Ingested inorganic nitrate (NO3-) has multiple effects in the human body including vasodilation, inhibition of platelet aggregation, and improved skeletal muscle function. The functional effects of oral NO3- involve the in vivo reduction of NO3- to nitrite (NO2-) and thence to nitric oxide (NO). However, the potential involvement of Snitrosothiol (RSNO) formation is unclear. We hypothesised that the RSNO concentration ([RSNO]) in red blood cells (RBCs) and plasma is increased by NO3--rich beetroot juice ingestion. In healthy human volunteers, we tested the effect of dietary supplementation with NO3--rich beetroot juice (BR) or NO3--depleted beetroot juice (placebo; PL) on [RSNO], [NO3-] and [NO2-] in RBCs, whole blood and plasma, as measured by ozone-based chemiluminescence. The median basal [RSNO] in plasma samples (n = 22) was 10 (5-13) nM (interquartile range in brackets). In comparison, the median values for basal [RSNO] in the corresponding RBC preparations (n = 19) and whole blood samples (n = 19) were higher (p < 0.001) than in plasma, being 40 (30-60) nM and 35 (25-80) nM, respectively. The median RBC [RSNO] in a separate cohort of healthy subjects (n = 5) was increased to 110 (93-125) nM after ingesting BR (12.8 mmol NO3-) compared to a corresponding baseline value of 25 (21-31) nM (Mann-Whitney test, p < 0.01). The median plasma [RSNO] in another cohort of healthy subjects (n = 14) was increased almost ten-fold to 104 (58-151) nM after BR supplementation (7 x 6.4 mmol of NO3- over two days, p < 0.01) compared to PL. In conclusion, RBC and plasma [RSNO] are increased by BR ingestion. In addition to NO2-, RSNO may be involved in dietary NO3- metabolism/actions.
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