4.8 Article

Differential DNA Damage Response of Peripheral Blood Lymphocyte Populations

期刊

FRONTIERS IN IMMUNOLOGY
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.739675

关键词

DNA damage response; peripheral blood lymphocyte subsets; mass cytometry; ataxia telangiectasia; cell cycle

资金

  1. German Else-Kroener-Fresenius (EKFS) foundation [2017_A57]

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DNA damage occurs constantly in cells due to internal and external factors, triggering different responses in lymphocyte subsets depending on maturation levels. These differences in DNA damage response play a role in cell survival and could be important for diagnostic purposes.
DNA damage occurs constantly in every cell triggered by endogenous processes of replication and metabolism, and external influences such as ionizing radiation and intercalating chemicals. Large sets of proteins are involved in sensing, stabilizing and repairing this damage including control of cell cycle and proliferation. Some of these factors are phosphorylated upon activation and can be used as biomarkers of DNA damage response (DDR) by flow and mass cytometry. Differential survival rates of lymphocyte subsets in response to DNA damage are well established, characterizing NK cells as most resistant and B cells as most sensitive to DNA damage. We investigated DDR to low dose gamma radiation (2Gy) in peripheral blood lymphocytes of 26 healthy donors and 3 patients with ataxia telangiectasia (AT) using mass cytometry. gamma H2AX, p-CHK2, p-ATM and p53 were analyzed as specific DDR biomarkers for functional readouts of DNA repair efficiency in combination with cell cycle and T, B and NK cell populations characterized by 20 surface markers. We identified significant differences in DDR among lymphocyte populations in healthy individuals. Whereas CD56(+)CD16(+) NK cells showed a strong gamma H2AX response to low dose ionizing radiation, a reduced response rate could be observed in CD19(+)CD20(+) B cells that was associated with reduced survival. Interestingly, gamma H2AX induction level correlated inversely with ATM-dependent p-CHK2 and p53 responses. Differential DDR could be further noticed in naive compared to memory T and B cell subsets, characterized by reduced gamma H2AX, but increased p53 induction in naive T cells. In contrast, DDR was abrogated in all lymphocyte populations of AT patients. Our results demonstrate differential DDR capacities in lymphocyte subsets that depend on maturation and correlate inversely with DNA damage-related survival. Importantly, DDR analysis of peripheral blood cells for diagnostic purposes should be stratified to lymphocyte subsets.

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