期刊
FRONTIERS IN IMMUNOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.687579
关键词
transcriptomics; sheep; lymph node; NK cells; Fasciola; IgE signalling
类别
资金
- Science Foundation Ireland [14/IA/2304]
- European Union [635408]
- Science Foundation Ireland (SFI) [14/IA/2304] Funding Source: Science Foundation Ireland (SFI)
This study analyzed the gene expression changes in ovine hepatic lymph nodes after infection with Fasciola hepatica using RNA sequencing. The findings suggest that the parasite may evade the host immune response by inhibiting pro-inflammatory pathways, NK cell activity, and IgE-mediated signaling among other key immunological pathways. Understanding these mechanisms will be crucial for the development of future vaccine prototypes against this parasite.
Fasciola hepatica is a trematode parasite responsible for major economic losses in livestock production, and is also a food-borne zoonotic agent in developing rural regions. For years, the immunoregulatory mechanisms employed by the parasite have hampered efforts to develop a successful vaccine candidate. Given that a comprehensive understanding of the immune response to infection is needed, we investigated the gene expression changes in ovine hepatic lymph nodes after experimental infection with F. hepatica. Lymph nodes from uninfected and infected animals were processed for RNA sequencing (RNA-seq) at 16 weeks post-infection. Comparison of groups revealed 5,132 differentially-expressed genes (DEGs). An inhibition of pro-inflammatory pathways, which has previously been described during fasciolosis, was evident in our data. However, other signals previously identified in ruminant peripheral blood mononuclear cells (PBMC) or liver tissue, such as activation of TGF-beta or apoptosis-related pathways were not detected. We found inhibition of some key immunological pathways, including natural killer (NK) cell activity and IgE-mediated signaling. These may point to additional some as yet unrecognized mechanisms employed by the parasite to evade the host immune response. Understanding these, and leveraging information from this and other omics studies, will be important for the development of future vaccine prototypes against this parasite.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据