期刊
FRONTIERS IN IMMUNOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.712402
关键词
hypoxia; oxygen sensing; CTLs; cytotoxic lymphocytes; quantitative proteomics; CD8 T cells
类别
资金
- Wellcome Trust [205023/Z/16/Z]
- Tenovus Scotland
- Biotechnology and Biological Sciences Research Council
- GlaxoSmithKline
- Biotechnology and Biological Sciences Research Council [BBS/E/B/000C0427, BBS/E/B/000C0428]
- Wellcome Trust [205023/Z/16/Z] Funding Source: Wellcome Trust
The study found that hypoxia increases the abundance of certain proteins in CTLs, while inhibiting cell proliferation and pro-inflammatory cytokine production. These findings are important for understanding CTL responses and the role of oxygen-sensing pathways in cellular control.
Metabolic and nutrient-sensing pathways play an important role in controlling the efficacy of effector T cells. Oxygen is a critical regulator of cellular metabolism. However, during immune responses T cells must function in oxygen-deficient, or hypoxic, environments. Here, we used high resolution mass spectrometry to investigate how the proteome of primary murine CD8(+) cytotoxic T lymphocytes (CTLs) is reconfigured in response to hypoxia in vitro. We identified and quantified over 7,600 proteins and discovered that hypoxia increased the abundance of a selected number of proteins in CTLs. This included glucose transporters, metabolic enzymes, transcription factors, cytolytic effector molecules, checkpoint receptors and adhesion molecules. While some of these proteins may augment the effector functions of CTLs, others may limit their cytotoxicity. Moreover, we determined that hypoxia could inhibit IL-2-induced proliferation cues and antigen-induced pro-inflammatory cytokine production in CTLs. These data provide a comprehensive resource for understanding the magnitude of the CTL response to hypoxia and emphasise the importance of oxygen-sensing pathways for controlling CD8(+) T cells. Additionally, this study provides new understanding about how hypoxia may promote the effector function of CTLs, while contributing to their dysfunction in some contexts.
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