期刊
APPLIED SCIENCES-BASEL
卷 11, 期 16, 页码 -出版社
MDPI
DOI: 10.3390/app11167730
关键词
rumen; odor; volatile fatty acids; methane; catalytic esterification
类别
资金
- Korea Environment Industry & Technology Institute (KEITI) - Korea Ministry of Environment (MOE) [RE202101439]
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea [318014]
- Cooperative Research Program for Agriculture Science & Technology Development Rural Development Administration, Republic of Korea [PJ01429702]
This study aimed to develop more accurate methods for measuring and specifying VFAs in bovine rumen, comparing the conventional method with an alternative CER method. The CER method showed improved accuracy in detecting C3+ VFAs, especially butyric acid, compared to the conventional method, providing quantitative proof of its superior performance. This suggests that the CER method is recommended for analyzing VFAs in rumen, particularly butyric acid and other C3+ VFAs.
Methane production from livestock farming is recognized as an important contributor to global GHGs. Volatile fatty acids (VFAs) found in bovine rumen may be utilized as a substrate for methanogens to form CH4, and thus improvement of quantitative VFA measurements can help facilitate greater understanding and mitigation of CH4 production. This study aims to contribute to the development of more accurate methods for the quantification and specification of VFAs in bovine rumen. The VFAs were analyzed using the conventional method and an alternative catalytic esterification reaction (CER) method. Substantial differences in the detected concentrations of the C3+ VFAs (chain length >= 3) were observed between both methods, especially for butyric acid. Evaluation of the sensitivity of both methods to detecting the VFA concentrations in standard solutions confirmed that the values resulting from the CER method were closer to the known concentrations of the standard solution than those from the conventional method. The results of this study provide the first quantitative proof to show the improved accuracy of the measurements of C3+ VFAs when using the CER method compared with the conventional method. Therefore, the CER method can be recommended to analyze the VFAs found in rumen, especially butyric acid and other C3+ VFAs.
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