期刊
AMB EXPRESS
卷 11, 期 1, 页码 -出版社
SPRINGER
DOI: 10.1186/s13568-021-01289-3
关键词
Variovorax paradoxus; BTEX; WGS; SBP encapsulation; Biobarrier
资金
- Ministry of Innovation and Technology [TKP2020-IKA-12]
- National Research, Development and Innovation office [2018-2.1.16-TET-IL-2018-00002, PD 128831]
- OTKA Young Researcher Excellence Programme [FK 134439]
- Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen (Nachwuchsgruppe Dr. Alexander Probst)
This study reports the applicability of Variovorax paradoxus strain BFB1_13 in the bioremediation of BTEX contaminated sites and found that it shows efficient biodegradation capabilities, especially towards benzene. Degradation pathways for BTEX were predicted based on gene annotation and metabolic analysis.
Here, we report and discuss the applicability of Variovorax paradoxus strain BFB1_13 in the bioremediation of BTEX contaminated sites. Strain BFB1_13 was capable of degrading all the six BTEX-compounds under both aerobic (O-2 conc. 8 mg l(-1)) and micro-aerobic/oxygen-limited (O-2 conc. 0.5 mg l(-1)) conditions using either individual (8 mg.l(-1)) or a mixture of compounds (similar to 1.3 mg.l(-1) of each BTEX compound). The BTEX biodegradation capability of SBP-encapsulated cultures (SBP-Small Bioreactor Platform) was also assessed. The fastest degradation rate was observed in the case of aerobic benzene biodegradation (8 mg l(-1) per 90 h). Complete biodegradation of other BTEX occurred after at least 168 h of incubation, irrespective of the oxygenation and encapsulation. No statistically significant difference was observed between aerobic and microaerobic BTEX biodegradation. Genes involved in BTEX biodegradation were annotated and degradation pathways were predicted based on whole-genome shotgun sequencing and metabolic analysis. We conclude that V. paradoxus strain BFB1_13 could be used for the development of reactive biobarriers for the containment and in situ decontamination of BTEX contaminated groundwater plumes. Our results suggest that V. paradoxus strain BFB1_13-alone or in co-culture with other BTEX degrading bacterial isolates-can be a new and efficient commercial bioremediation agent for BTEX contaminated sites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据