期刊
ADVANCED SCIENCE
卷 8, 期 18, 页码 -出版社
WILEY
DOI: 10.1002/advs.202100964
关键词
electrical stimulation; mesenchymal stromal cells; osteogenesis; pulsed current; rejuvenation; triboelectric nanogenerator
资金
- National Natural Science Foundation of China (NSFC) [82072505, 81772401, 81902260, 61804103]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [18KJA535001]
- Guangdong Basic and Applied Basic Research Foundation for Distinguished Young Scholars [2020B1515020027]
- Guangzhou Science and Technology Bureau [202002020070, 202102010181, 202102010007]
- Fundamental Research Funds for the Central Universities [19ykpy108]
- Guangdong Science and Technology Department [2020B1212060018, 2020B1212030004]
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- 111 Project
- Joint International Research Laboratory of Carbon-Based Functional Materials and Devices
The study introduces a new method for rejuvenating senescent bone marrow mesenchymal stromal cells (BMSCs) using pulsed triboelectric stimulation. This approach reverses the senescence phenotypes of aged BMSCs, enhances their proliferation and pluripotency, and rejuvenates them by enhancing MDM2-dependent p53 degradation through pulsed triboelectric stimulation by P-TENG.
Stem cell senescence contributes to stem cell exhaustion and drives various aging-associated disorders. However, strategies to rejuvenate senescent stem cells are limited. The present study proposes an approach based on triboelectric stimulation to rejuvenate senescent bone marrow mesenchymal stromal cells (BMSCs) by fabricating a pulsed triboelectric nanogenerator (P-TENG) that can produce stable pulsed current output unaffected by the triggered frequency. The senescence phenotypes of aged BMSCs are reversed by triboelectric stimulation at 30 mu A at 1.5 Hz. Triboelectric stimulation enhances the proliferation of aged BMSCs and increases their pluripotency and differentiation capacity. Additionally, mechanistic investigations reveal that pulsed triboelectric stimulation by P-TENG rejuvenates senescent BMSCs by enhancing MDM2-dependent p53 degradation, which is demonstrated by loss-of-function studies of MDM2 and p53. Overall, this study identifies a new approach for the rejuvenation of senescent BMSCs and describes a promising therapeutic intervention for many diseases associated with aged BMSCs.
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