期刊
ADVANCED SCIENCE
卷 8, 期 17, 页码 -出版社
WILEY
DOI: 10.1002/advs.202101433
关键词
intravital two-photon microscopy; magnetic resonance imaging; microglia; macrophage; multimodal nanoprobe; neuroinflammation; stroke
资金
- French national research agency (ANR) project NanoBrain [ANR-15-CE18-0026-01]
- RHU MARVELOUS of University Claude Bernard Lyon 1 (UCBL), within the Investissements d'Avenir program [ANR16-RHUS-0009]
This study proposes and validates a preclinical in vivo MRI tool to monitor neuroinflammation following ischemic stroke, based on injection of a novel multimodal nanoprobe designed for internalization by phagocytic cells. The study confirms efficient internalization of NanoGd by microglia and shows hypointense signals in the ischemic lesion using in vivo MRI with NanoGd injection in a mouse model. Additionally, the study demonstrates NanoGd internalization by activated phagocytic cells through both intravital microscopy and ex vivo analysis.
The purpose of this study is to propose and validate a preclinical in vivo magnetic resonance imaging (MRI) tool to monitor neuroinflammation following ischemic stroke, based on injection of a novel multimodal nanoprobe, NanoGd, specifically designed for internalization by phagocytic cells. First, it is verified that NanoGd is efficiently internalized by microglia in vitro. In vivo MRI coupled with intravenous injection of NanoGd in a permanent middle cerebral artery occlusion mouse model results in hypointense signals in the ischemic lesion. In these mice, longitudinal two-photon intravital microscopy shows NanoGd internalization by activated CX3CR1-GFP/+ cells. Ex vivo analysis, including phase contrast imaging with synchrotron X-ray, histochemistry, and transmission electron microscopy corroborate NanoGd accumulation within the ischemic lesion and uptake by immune phagocytic cells. Taken together, these results confirm the potential of NanoGd-enhanced MRI as an imaging biomarker of neuroinflammation at the subacute stage of ischemic stroke. As far as it is known, this work is the first to decipher the working mechanism of MR signals induced by a nanoparticle passively targeted at phagocytic cells by performing intravital microscopy back-to-back with MRI. Furthermore, using a gadolinium-based rather than an iron-based contrast agent raises future perspectives for the development of molecular imaging with emerging computed tomography technologies.
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