期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 7, 期 9, 页码 4602-4613出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.1c00862
关键词
matrix stiffness; mesenchymal stem cells; proliferation; RNA-seq; alginate
资金
- National Natural Science Foundation of China [21734002, 51825302, 52021004, 31700827]
- State Key Project of Research and Development [2016YFC1100300, 2017YFB0702603]
- Technology Innovation and Application Development Program of Chongqing [cstc2019jscx-msxmX0236]
The increased matrix stiffness was found to promote the proliferation-related signaling of mesenchymal stem cells (MSCs), and this process depended on the increased cortex tension caused by the activation of RAS and myosin II.
The mechanical properties of the natural extracellular matrix (ECM) change extensively, but these specific properties provide a relatively stable environment for resident cells. Although the effect of matrix stiffness on cell functions has been widely studied, the molecular mechanism was still not fully understood. Matrix stiffening is a common phenomenon in tissue damaging processes. To explore the effect of the increase in local matrix stiffness on cell behaviors, a three-dimensional (3D) cell culture system with a tunable modulus but constant other physical parameters was constructed by the alginate hydrogel with different molecular weights and cross-linking degrees. By using this culture system, the transcriptome response of mesenchymal stem cells (MSCs) to matrix stiffness was explored. Furthermore, a finite element model was developed to simulate the interaction between cells and the matrix. Results revealed that the increased matrix stiffness promoted the proliferation-related signaling of MSCs, and this process depended on the increased cortex tension caused by the activation of RAS and myosin II.
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