期刊
STEM CELL REPORTS
卷 16, 期 9, 页码 2351-2363出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2021.08.002
关键词
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资金
- Ministerio de Economa y Competitividad [SAF2017-82597-R, PID2020-114846RB-I00]
- Junta de Extremadura [GR18006, IB160210, IB20014]
- European Union FEDER program
- Spanish Ministry of Science, Innovation and University
Aryl hydrocarbon receptor (AhR) regulates pluripotency factors OCT4/POU5F1 and NANOG in early mouse embryogenesis, playing a crucial role in controlling pluripotency and differentiation.
Mammalian embryogenesis is a complex process controlled by transcription factors that regulate the balance between pluripotency and differentiation. Transcription factor aryl hydrocarbon receptor (AhR) regulates OCT4/POU5F1 and NANOG, both essential controllers of pluripotency, stemness and early embryo development. Molecular mechanisms controlling OCT4/POU5F1 and NANOG during embryo -genesis remain unidentified. We show that AhR regulates pluripotency factors and maintains the metabolic activity required for proper embryo differentiation. AhR-lacking embryos (AhR(-/-)) showed a pluripotent phenotype characterized by a delayed expression of tro-phectoderm differentiation markers. Accordingly, central pluripotency factors OCT4/POU5F1 and NANOG were overexpressed in AhR(-/-) embryos at initial developmental stages. An altered intracellular localization of these factors was observed in the absence of AhR and, importantly, Oct4 had an opposite expression pattern with respect to AhR from the two-cell stage to blastocyst, suggesting a negative regulation of OCT4/POU5F by AhR. We propose that AhR is a regulator of pluripotency and differentiation in early mouse embryogenesis.
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