期刊
NANOMATERIALS
卷 11, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/nano11092457
关键词
photoacoustic imaging; polypyrrole; nanoparticles; contrast agents
类别
资金
- Czech Science Foundation [18-05200S]
- Ministry of Education, Youth and Sports of the Czech Republic [LM2018129]
- National Sustainability Program II [BIOCEV-FAR LQ1604]
- Charles University [SVV 260 519/2020]
- European Regional Development Fund [CZ.02.01./0.0./0.0./16_013/0001775]
The study explored the properties of polypyrrole nanoparticles in photoacoustic imaging, revealing the impact of oxidant ratios and particle size on photoacoustic signal intensity. In vivo experiments on coated nanoparticles showed promising results, indicating their potential as contrast agents for photoacoustic imaging.
Photoacoustic imaging, an emerging modality, provides supplemental information to ultrasound imaging. We investigated the properties of polypyrrole nanoparticles, which considerably enhance contrast in photoacoustic images, in relation to the synthesis procedure and to their size. We prepared polypyrrole nanoparticles by water-based redox precipitation polymerization in the presence of ammonium persulphate (ratio nPy:nOxi 1:0.5, 1:1, 1:2, 1:3, 1:5) or iron(III) chloride (nPy:nOxi 1:2.3) acting as an oxidant. To stabilize growing nanoparticles, non-ionic polyvinylpyrrolidone was used. The nanoparticles were characterized and tested as a photoacoustic contrast agent in vitro on an imaging platform combining ultrasound and photoacoustic imaging. High photoacoustic signals were obtained with lower ratios of the oxidant (nPy:nAPS >= 1:2), which corresponded to higher number of conjugated bonds in the polymer. The increasing portion of oxidized structures probably shifted the absorption spectra towards shorter wavelengths. A strong photoacoustic signal dependence on the nanoparticle size was revealed; the signal linearly increased with particle surface. Coated nanoparticles were also tested in vivo on a mouse model. To conclude, polypyrrole nanoparticles represent a promising contrast agent for photoacoustic imaging. Variations in the preparation result in varying photoacoustic properties related to their structure and allow to optimize the nanoparticles for in vivo imaging.
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