期刊
NANOMATERIALS
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/nano11082072
关键词
cellulose nanocrystals; cationic; immunomodulator; hemolysis; cytotoxicity
类别
资金
- National Science Foundation [1703890]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1703890] Funding Source: National Science Foundation
The study investigated the impact of cationic cellulose nanocrystals on human blood and endothelial cells, finding that these nanoparticles did not cause significant adverse effects at certain concentrations, which may be beneficial for potential biomedical applications.
An emerging interest regarding nanoparticles (NPs) concerns their potential immunomodulatory and pro-inflammatory activities, as well as their impact in the circulatory system. These biological activities of NPs can be related to the intensity and type of the responses, which can raise concerns about adverse side effects and limit the biomedical applicability of these nanomaterials. Therefore, the purpose of this study was to investigate the impact of a library of cationic cellulose nanocrystals (CNCs) in the human blood and endothelial cells using cell-based assays. First, we evaluated whether the cationic CNCs would cause hemolysis and aggregation or alteration on the morphology of red blood cells (RBC). We observed that although these nanomaterials did not alter RBC morphology or cause aggregation, at 24 h exposure, a mild hemolysis was detected mainly with unmodified CNCs. Then, we analyzed the effect of various concentrations of CNCs on the cell viability of human umbilical vein endothelial cells (HUVECs) in a time-dependent manner. None of the cationic CNCs caused a dose-response decrease in the cell viability of HUVEC at 24 h or 48 h of exposure. The findings of this study, together with the immunomodulatory properties of these cationic CNCs previously published, support the development of engineered cationic CNCs for biomedical applications, in particular as vaccine nanoadjuvants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据